MCDB 427 Lecture Notes - Lecture 4: Autoradiograph, Affinity Chromatography, Electric Field
21 Dec 2016
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Uses argose to generate a gel and we generate wells which is where we apply our nucleic acid sample. Separation methods: ways to separate nucleic acids from one another. Label tracer methods: ways we label particular macromolecules; how we identify or detect specific nucleic acids. The goal here is to separate nucleic acids by size. These are the (cid:885) different types of techniques that we"ll learn today. When we talk about size, we"re really talking about length. Since nucleic acid is negative charged, it"ll flow to the positive end. After the electrophoresis takes place, we still can"t see the bands with our eyes. Because when ethium bromide goes into the dna, if there is more dna there, you"ll. The marker lane has four different framents (cid:523)on this figure(cid:524) but they don"t all have. The gel inhibits longer dna fragments to migrate whereas the small ones can pass through more easily.
