MCDB 427 Lecture Notes - Lecture 19: Prokaryotic Large Ribosomal Subunit, Ribose, World Federation Of Trade Unions

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purplebeaver856

21 Dec 2016

School

U of M

Department

Molecular, Cellular and Developmental Biology

Course

MCDB 427

Professor

Janine Maddock

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Document Summary

The first trna that binds is the initiating aminoacyl-trna fmet trna-fmet in the p site. We bring in another aminoacyl-trna based on the codon in the a site. It comes in through a complex with ef-tu and gtp site. Now we have to move this dipeptide over to the next side (p site) This is looking at the amino acids in the ribosome. Now the ribosome does it"s function and transfer the peptide from the p site to the a. The e site doesn"t have any amino acids. We"re only looking at the (cid:885)" of the trna which all end with cca. We see that there are amino acids on both the p and the a site. What peptidyl transferase does is that it transfers the p site peptide or polypeptide chain to the amino acid in the a site. So how do you prove that there are different sites, fmet goes to the p site and not the.

Related Questions

Given the wobble rules, what is the fewest number of tRNAs necessary to insert the amino acid Leu into a peptide, and what is the anticodon for each tRNA? There are six codons that specify Leu: UUA, UUG, CUU, CUC, CUA, CUG. Keep in mind all sequences in this question are written 5' to 3'

A) two, with anticodons IAA and GAG

B) two, with anticodons UAA and IAG

C)three, with anticodons IAA, GAG, and UAG

D) three, with anticodons UAA, GAG, and UAG

E)three, with anticodons UAA, IAG, and UAG

A researcher isolates a mutant variant of the bacterial translation factor EF-G. The mutation allows proper folding of the protein and binding of GTP, but does not allow GTP hydrolysis. At what stage would translation be blocked by this mutant protein?

A)Binding of fMet-tRNA^fMet to P site.

B) Second aminoacyl-tRNA binding to the ribosomal A site.

C) Formation of peptide bond between amino acids.

D) Movement of ribosome along the mRNA to allow binding of a new EF-TuâtRNA in A site.

Translation factor EF-Tu uses GTP hydrolysis to ensure thecorrect amino-acyl tRNA is docked into the ribosome âAâ site. Whatbe the consequence of a mutation in EF-Tu that causes the proteinto hydrolyze more quickly? Explain your answer.

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22. There are 20 amino acids and 64 possible codons. Which of the following is closer to the actual number of different tRNA molecules in a cell?

A. 4

B. 16

C. 20

D. 61

E. 64

23. There are 20 amino acids and 64 possible codons. How many different eukaryotic aminoacyltRNA synthetases are there? A. 1

B. 16

C. 20

D. 61

E. 64

24. Which way are the amino acid and tRNA attached in a charged tRNA?

A. Amino acid-5'tRNA3'-AMP B

. Amino acid-AMP-3'tRNA

C. AMP-amino acid-3'tRNA

D. Amino acid-3'tRNA5'-AMP

E. Amino acid-AMP-5'tRNA

25. The anticodon that recognizes the initiation codon in translation is

A. 5' AUG 3'

B. 3' AUG 5'

C. 5' ACU 3'

D. 5' UAC 3'

E. 3' UAC 5'

26. The first amino acid incorporated into a growing polypeptide

A. In bacteria is methionine and it is also methionine in eukaryotes

B. In bacteria is tryptophan, while it is methionine in eukaryotes

C. In bacteria is N-formyl methionine, while it is methionine in eukaryotes

D. In bacteria is N-formyl methionine and it is also N-formyl methionine in eukaryotes

E. In bacteria is methionine, while it is N-formyl methionine in eukaryotes

27. In E. coli the IF3 forces tRNAf Met to bind with

A. The A site of the ribosome

B. The P site of the ribosome

C. The E site of the ribosome

D. IF1

E. IF2

MCDB 427 Lecture Notes - Lecture 19: Prokaryotic Large Ribosomal Subunit, Ribose, World Federation Of Trade Unions

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