Histone Dynamics & Histone Modifications

2 Pages
Unlock Document

Biochemistry & Molecular Bio.
David Gross

Feb 19 Notes • Histones are dynamic o DNAwraps & unwraps from histones approximately 4x per second o DNA-binding proteins bind when nucleosome unwrapped for 10-50 milliseconds o DNAunwrapped 2-10% of time (can tell by FRET) • Histones promote DNAwrapping & unwrapping o DNAmore pliable than previously thought • nucleosomes can slide along chromosome o ATP-dependent remodeling complexes assist in this o with histone chaperones they can exchange histone components or even entire core  histone chaperones keep histones soluble until recruited into nucleosomes • measuring histone dynamics o distinguishing a pool of histones o determining how fast this pool exchanges w/ other histones o this is done by marking histones o can use Chip-SEQ against specific tags to determine o specific regions of chromosomes are more dynamic than others • histones can be modified o N-terminus exposed even when in nucleosomes o lysine can be acetylated or mono-, di-, or trimethylated (competing reactions) o serine can be phosphorylated o the N-terminu
More Less

Related notes for BIOCHEM 524

Log In


Join OneClass

Access over 10 million pages of study
documents for 1.3 million courses.

Sign up

Join to view


By registering, I agree to the Terms and Privacy Policies
Already have an account?
Just a few more details

So we can recommend you notes for your school.

Reset Password

Please enter below the email address you registered with and we will send you a link to reset your password.

Add your courses

Get notes from the top students in your class.