BIOLOGY 151 Lecture Notes - Lecture 6: Rna Polymerase Ii, Pribnow Box, Protein Subunit

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14 Feb 2018

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Initiation and termination of transcription: transcription in initiated at a promoter sequence and ends at a terminator sequence. The tra(cid:374)script is sy(cid:374)thesized i(cid:374) a 5" to 3" directio(cid:374): both dna strands serve as templates for transcription. Initiation is the first phase of transcription: rna polymerase cannot initiate transcription on its own, sigma is a protein subunit that must first bind to the polymerase. Bacterial promoters: bacterial promoters, are compromised of 40-50 base pairs, have two key regions = -10 box and -35 box, the -10 box (pribnow box) Is found 10 bases upstream from the transcription start site (the +1 site: consists of the sequence tataat, the -35 box. Is 35 bases upstream from the +1 site: consists of the sequence ttgaca, all bacterial promoters have a -10 box and a -35 box, the remainder of the promoter sequence varies. Each type allows rna polymerase to bind to a different type of promoter therefore a different kind of gene.

Related Questions

Order the following choices to examine the process of transcription.

- RNA polymerase moves along the template strand of DNA adding complementary RNA nucleotides, extending the mRNA.

-RNA polymerase binds at the promoter region just upstream from the gene.

-RNA polymerase unwinds the DNA at the promoter site and begins to transcribe the template strand of DNA.

-Transcription continues until a termination site in the DNA is reached.

-The mRNA transcript is released.

During transcription in eukaryotes, a type of RNA polymerase called RNA polymerase II moves along the template strand of the DNA in the 3'â5' direction. However, for any given gene, either strand of the double-stranded DNA may function as the template strand. Which of the following initially determines which DNA strand is the template strand, and therefore in which direction RNA polymerase II moves along the DNA?

A. the location of specific proteins (transcription factors) that bind to the DNA

B. the specific sequence of bases along the DNA strands

C. the location along the chromosome where the double-stranded DNA unwinds

D. the position of the geneâs promoter on the chromosome

E. which of the two strands of DNA carries the RNA primer

Which type of RNA is unique to eukaryotes?

		mRNA
		rRNA
		tRNA
		snRNA
		Its a trick, all cells have all four types of RNA.

1 points

QUESTION 2

The region of DNA to which RNA polymerase binds to initiate transcription is the:

		origin
		promoter
		enhancer
		none of the above are correct

1 points

QUESTION 3

Holoenzyme:

		is RNA polymerase core enzyme.
		is sigma factor.
		is core plus sigma factor.
		none of the above are correct.

1 points

QUESTION 4

Which of these is formed FIRST when RNA polymerase binds to a promoter?

		The closed promoter complex
		The open promoter complex
		A rho-independent terminator
		none of the above are correct.

1 points

QUESTION 5

A hairpin loop followed by a run of Us:

		is the sequence formed in the sense strand of a gene using a rho-independent terminator.
		is the sequence recognized by the rho protein.
		is the structure formed in RNA that causes rho-independent termination.
		is formed immediately after the open promoter complex is formed.

1 points

QUESTION 6

RNA polymerase:

		reads the template strand of the DNA in the 3' to 5' direction.
		synthesizes the RNA in the 5' to 3' direction.
		can begin transcription without a primer.
		all of the above are correct.

1 points

QUESTION 7

Which of the following statements is FALSE?

		Bacteria like E. coli have only one type of sigma factor.
		RNA polymerase has no proofreading activity.
		The promoter of a gene is upstream of the RNA-coding region.
		Bacterial transcriptional terminators are copied into RNA to cause termination.

1 points

QUESTION 8

If the template strand of a gene has the sequence 5' ATGCGAT 3', then:

		the RNA produced will have the sequence 5' AUGCGAU 3'.
		the RNA produced will have the sequence 5' UAGCGUA 3'.
		the RNA produced will have the sequence 5' AUCGCAU 3'.
		None of the above are correct.

1 points

QUESTION 9

The sense strand:

		is the lagging strand
		is also the template strand.
		always goes left to right.
		none of the above are correct.

1 points

QUESTION 10

Which of these is transcribed but not translated?

		mRNA
		tRNA
		rRNA
		all of the above
		tRNA and rRNA are transcribed but not translated.

please help i am a bit confused

BIOLOGY 151 Lecture Notes - Lecture 6: Rna Polymerase Ii, Pribnow Box, Protein Subunit

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