BIOLOGY 285 Lecture Notes - Lecture 6: Transcription Factor, Tyrosine, Rna Interference
16 Nov 2020
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Document Summary
Compare and contrast in mitochondrial/ er import: signal sequences are n-terminus, protein is unfolded when imported (single stranded, must fold in organelle, n-terminal signal sequence is cleaved off. Crispr crispr rna bacterial defense mechanism against dna, but can be used to edit genomes snrna spliceosome rna. Proteins that bind dna: sequence specific dna binding, non-sequence specific dna bindings. It binds ~800 promoters in e. coli each promoter contains sigma 70 binding sequence with more or less affinity. Transcriptional regulation: trp operon in e. coli, sigma factors in e. coli general tf, sigma 70 housekeeping sigma in e. coli. Consensus sequence frequency analysis mrna with multiple proteins coded within polycistronic mrna code 1 protein (monocistronic) Tryptophan amino acids: e. coli can get from environment or synthesize it, trp operon encodes 5 proteins (enzymes) required for trp biosynthesis. Mt: stabilizing mts will stabilize the overall cell that will divide properly.
