CHEN 3701 Lecture Notes - Lecture 37: Chemostat, Commission On Science And Technology For Development
17 Nov 2015
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Maximize biomass productivity in a chemostat if assume monod kinetics if very well controlled, can operate very close to this max dif not as good, go a bit below it. Consider cell death and/or endogenous metabolism for steady state for sterile feed. Chen3701_biomolecularengineering page 1 but we do have death. Assume steady state: to find free substrate concentration that has not been consumed yet. High microbial cell densities require high carbon source (ex: glucose, etc. ) concentrations (~10% by mass) Consumption of that substrate (that you feed to the cells) quickly drops that concentration below optimal. -> can fix this by putting even more carbon source to startbut can"t go too high. Yet starting high is problematic since very high carbon concentrations yield metabolic byproduct toxisity, osmotic stress in extracellular environment, and viscous culture. -> do substrate balance similar to chemostat but now outflow negligible product formation, steady substrate concentration differential = 0, last term = 0 for negligible product formation.
