CHEM 2121 Lecture Notes - Meke, Betta, Apen
Document Summary
To get more closely spaced peaks (but with still good resolution) increase t like this. Reproducability and retention time are worse when you"re constantly changing t and keep reequilibrating. We want to be able to do this repetitively t programming needs more maintanence than isothermal. Usually runs on its ownno person operates it. *if don"t know how long to wait, can make t very high and cool down as needed. *want to ramp up t in step curve b/c if do constantly, can ruin resolutionwant closer peaks and good resolution. -> main goal is to maximize resolution in minimal amount of time. Don"t really decrease t ever b/c never helps youif you"re going to raise it just to decrease itnot beneficial. High performance liquid chromatography (hplc) (the p may also be for pressure) Only go here when gc doesn"t workgc is more easy instrumentally, more efficient separation. If volatile, want gc; if gases, want gc.
