CHEM 2121 Lecture Notes - Analyte, Rieti, Opata Language
Document Summary
Now talking about quantitative analysis looking at mass spec w/ chromatography. Our analyte here is a mixture of herbicides in river water. Doing uv/vis detection (figure (a)) --> uv detection @ 240 mm. But uv detector"s not as selectiveanyone can absorb and thus will be detected: using mass spec using ion spray, reconstructed total ion chromatogram. -sum total ions over m/z range: selected ion monitoring (this is were we really see capability of this method) In mass filter section, m/z = 312 in part c, monitor a specific m/z of a known ion of the analyte of interest. Reduces background noiselittle else yields m/z = 312 (unique to the one we"re looking for) More relevant data pointsthere"s a lot of other stuff we don"t need/care for. If doing internal standard, need one that"s at 312hard to choose that. If don"t know about your sample, hard to do this.
