Class Notes at University of New England

Lecture 11 Purifying, Detecting Characterizing Proteins A protein must be puried to determine its structure and mechanism of action Molecules, including proteins, can be separated from other molecules based on differences...

Genetics 500: Molecular Biology Introduction LECTURE 12 - Immunoblots Immunoblotting, also called Western blotting, combines the resolving power of gel electrophoresis with the specificity of antibodies. This multistep pro...

Lecture 13 Molecular Mass Protein Sequencing Molecular mass can be determined by matrix- assisted laser desorptionionization timeof- flight (MALDI-TOF) mass spectrometry. Step 1: In a MALDI-TOF mass spectrometer, pulses of...

Lecture 6 Protein Design Common principle in previously listed types of function is ability to bind to things (other proteins, ions, other macromolecules....ligands) Binding often involves conformational changes Binding is...

Lecture 9 Regulating Protein Function Alter steady state amount Alter rate of synthesis Alter rate of degradation Alter activity of protein Alter non-covalent, including allosteric, interactions Alter covalent modications ...

Lecture 10 : Types of Regulation G-Protein Switches (Regulation) Large family of GTP binding and hydrolyzing proteins where a protein conformational change is caused by GTP binding GAP proteins control how long G protein r...

Lecture 7 Basics of Enzymes Proteins that catalyze chemical reactionsthe making and breaking of covalent bondsare called enzymes, and the ligands of enzymes are called substrates. Another form of catalytic macromolecule in...

Lecture 8 - Antibodies Protein Complexes Antibodies stabilize the ground state Enzymes stabilize the transition state Some transition states are stable enough that they can be trapped and used as antigens The antibodies p...

Lecture 2 - Structure Motifs A particular combination of two or more secondary structures that form a distinct three- dimensional structure is called a structural motif when it appears in multiple proteins Structural motif...

LECTURE 5 Correcting Misfolded Proteins Although a small protein like ribonuclease can fold spontaneously, even it is slow by biological standards In the cell, protein folding is assisted by chaperones and chaperonins Chap...

Lecture 1 - Protein Structure These functions summarize the main functions of the proteins Proteins develop many of these diverse functions through exploiting a few simple activities: Most fundamentally, proteins bindto on...

Lecture 4 - Number of Proteins Correcting Misfolded Proteins Although a small protein like ribonuclease can fold spontaneously, even it is slow by biological standards In the cell, protein folding is assisted by chaperones...

Lecture 3 Protein Evolution Number of Proteins Molecular chaperones, which bind to a short segment of a protein substrate and stabilize unfolded or partly folded proteins, thereby preventing these proteins from aggregating...

Winter 2016/17 Tentative weather prospects 27/06/2016 UPDATE 15/09/16 now online UK winter 2016/17 weather forecast indicators It's a long way off but are there any early pointers to how winter 2016/17 may turn ou...