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Class Notes for Biochemistry at University of Iowa

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BIOC 3110 Lecture Notes - Lecture 41: Transferrin Receptor, Ferritin, Transferrin
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Lecture 41: Mechanism of Protein Synthesis Recombinant DNA techniques Transferrin-Ferritin receptor system as an example of protein translation regulation  Predict the consequence of putting an IRE-BP recognition ...

Biochemistry
BIOC 3110
marcwold
BIOC 3110 Lecture Notes - Lecture 40: Start Codon, World Federation Of Trade Unions, Gtpase
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Lecture 40: Protein Synthesis  Intro: Carboxyl terminal on first amino acid forms an ester bond with the tRNA, which is highly unstable; the incoming amino acid’s NH3 end attacks the ester linkage and...

Biochemistry
BIOC 3110
marcwold
BIOC 3110 Lecture Notes - Lecture 39: Ribose, Peptide, Base Pair
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Lecture 39: The Genetic Code  Two key components of genetic code: tRNAs (readers of the code because these RNAs are the link between the mRNA and amino acids); and Amino acyl tRNA synthetases (readers...

Biochemistry
BIOC 3110
marcwold
BIOC 3110 Lecture Notes - Lecture 38: Rna Splicing, Rna Polymerase Ii, Ribonuclease Iii
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Lecture 38: RNA Processing in Eukaryotes  Each RNA type has a different type of processing  What classes of RNA-protein complexes contribute to the maturation of RNAs in eukaryotic cells? ...

Biochemistry
BIOC 3110
marcwold
BIOC 3110 Lecture Notes - Lecture 37: Transfer Rna, Arginine, Transcription Factor Ii D
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Lecture 37: Eukaryotic Gene Expression  Which RNAs are transcribed by each eukaryotic RNA polymerase? o RNAP I- located in nucleolus, transcribes ¾ ribosomal RNAs o RNAP II-located in nucleopl...

Biochemistry
BIOC 3110
marcwold
BIOC 3110 Lecture 36: RNA synthesis and Regulation in Prokaryotes
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Lecture 36: RNA Synthesis and Regulation in Bacteria RNAP works by reading the template DNA strand (aka noncodinganti sense strand) in the 3 5 direction, and produces mRNA in the 5 3 direction No primer required for RNAP I...

Biochemistry
BIOC 3110
marcwold
BIOC 3110 Lecture Notes - Lecture 35: Holliday Junction, Phosphodiesterase, Ames Test
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Lecture 35: DNA repair Types of mutations: Single, base-pair changes, deletion of nucleotides, insertion of nucleotides, chromosomal rearrangements • How are mutations made? o DNA Replication Mutations: ...

Biochemistry
BIOC 3110
marcwold
BIOC 3110 Lecture Notes - Lecture 34: Dna Polymerase Iii Holoenzyme, Dna Polymerase I, Replication Protein A
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Lecture 34: DNA replication • What seven activities are requirements for DNA replication? o DNA polymerase III ▪ Require: ▪ a template strand to select incoming nucleo...

Biochemistry
BIOC 3110
marcwold
BIOC 3110 Lecture Notes - Lecture 33: Phosphodiester Bond, Hydrogen Bond, Thymine
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Lecture 33: DNA • Structures of bases: o DNA is polymer of 4 monomers o Monomer made of base, sugar, and phosphate (at 3’ spot) o They are connected through phosphodiester bonds ...

Biochemistry
BIOC 3110
marcwold
BIOC 3110 Lecture Notes - Lecture 32: De Novo Synthesis, Carbamoyl Phosphate, Aspartate Carbamoyltransferase
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Lecture 32: Nucleotide Metabolism We previously talked about how amino acids are made; amino acids are also required to make a variety of biomolecules, one being de novo synthesis of nucleotides • Be able to recogn...

Biochemistry
BIOC 3110
marcwold
BIOC 3110 Lecture Notes - Lecture 31: Glutamate Dehydrogenase, Nitrogen Fixation, Nitrogenase
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Lecture 31: Amino Acid Synthesis To make proteins, bases, etc. , cells have to have amino acids that are either made or supplied by the diet N2NH3amino acid • What is nitrogen fixation and why is it important? ...

Biochemistry
BIOC 3110
marcwold
BIOC 3110 Lecture Notes - Lecture 30: Glutamine Synthetase, Carbamoyl Phosphate, Glutamate Dehydrogenase
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Lecture 30: Amino Acid Degradation + Urea Cycle • Proteins and cellular proteins are degraded to amino acids • AA structure: NH3, COOH, R, H • Do cells have a storage form for excess amino acids? ...

Biochemistry
BIOC 3110
marcwold
BIOC 3110 Lecture Notes - Lecture 29: Membrane Protein, Acetoacetyl-Coa, Oligosaccharide
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Lecture 29: Lipid Synthesis What are the features that are common to or unique to the generation of storage, membrane, or signaling lipids? o Storage lipidtriacylglycerol Precursor: phosphatidate made from adding 3 fatty a...

Biochemistry
BIOC 3110
marcwold
BIOC 3110 Lecture Notes - Lecture 28: Methylene Group, Aspirin, Acetaldehyde
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Lecture 28: Fatty Acid Synthesis Fatty acid synthesis occurs when you have lots of energy, lots of carbon, which says there is spare to make fatty acids, to use to make membranes. What are the building blocks for fatty aci...

Biochemistry
BIOC 3110
marcwold
BIOC 3110 Lecture Notes - Lecture 27: Dihydroxyacetone, Acyl Coa Dehydrogenase, Lysine
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Lecture 27: Fatty Acid Degradation 3 stages of fatty acid processing: o 1. Degrade triacylglycerol to release fatty acids and glycerol into the blood for transport to energy requiring tissues breaking from lipid droplet o ...

Biochemistry
BIOC 3110
marcwold

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