BIOL 483 Lecture Notes - Lecture 65: Long Non-Coding Rna, Rna-Dependent Rna Polymerase, Agos
Document Summary
2 classes: ago like, piwi like, acts in sirna pathway, acts in pirna pathway. Piwi domain binds 5" end to small rna. Paz domain binds 3" end to small rna guide strand. Some agos cut mirna (slicer activity) and other end of protein folds back with piwi domain to contact other strand of sirna. Hmt to make h3k9me2/3 (silencing marks), clr4 silencing based on repeat from which sirna is generated. At repeats, rna pol works on other strand of dna -> lncrna. Ago1 = contains red guide strand dna complementary to rna to help stabilise hmt by. Rna components and chp! (1 of 3 hp1 proteins) Once there is h3k9me3 -> recruitment of swi6 -> heterocrhomatin formation. Chp2: hek9me3 allows for swi6 (or hp1) and hdac (or clr3) binding, locus of interest is silenced. Rdrc complex = rna dependent rna polymerase complex, generates more sirna for amplification.
