BIOL 483 Lecture Notes - Lecture 76: Cryogenic Transmission Electron Microscopy, Zinc Finger Nuclease, Chromosome
Document Summary
In kleinfelters there"s an extra dosage of x linked genes, still have extra genes that escape x inactivation so dosage is imbalance compared to xy individual. How to use technology of a lncrna that silences entire chromosome, how to correct dosage imbalance. In downsyndrome there"s 3 copies of chromsoome 21. They took downsyndrome patient fibroblast and reprogrammed them to ips cells and thne used zinc finger nucleases to introduce an inducible transgene into chromosome. 21 and when they they add dox it stimulates x inactivatio nand form a barr body and become transcriptionally silenced. Looked at all chromosome, chromosome 21 had huge reduction in trnsacriptional activity, use of barr body worker. Shut off most of it but enough to make substantial corrections in expression of chromosome 21. How does activity of prc2 get stimulated by substrate, in particular by chromosome and nucleosomes that are close together.
