BIOL 483 Lecture Notes - Lecture 92: Red, Tads, Heterochromatin
Document Summary
Compartments are made of compartment a or b. In compartmnet b = closed chromatin, at periphery of nucleus, hugs nuclear lamina. Compartment a = inside nucleus, active chromatin, things that are replicating. If we big further into compartments/zooming into shorter length of dna, we see tads. Tads in terms of bulk sequencing, these are largely stable - tads when you look at single cells can be heterogeneous/ unstable (but in this case, it"s stable for bullk populations) - stable acrss bulk populations/cells. Boundaries formed by insulator boundaries, which contain ctcf and cohesin proteins via the loop extrusion model (ctcf binding motif binds to ctcf and cohesin is rubber band holding everything tgt) > some other proteins at boundaries are rad21, rna pol. Tads always include at least 1 gene, so they include many enhancer/promoter interactions in unit of transcriptional activity. When we dig in more into tads, we get sub-tads (more dynamic, flexible, tads within tads)
