BIOL 483 Lecture Notes - Lecture 90: Sister Chromatids, Nucleolus, Condensin
Document Summary
Nuclear structure plays key role in forming a (active) compartments that interact with each other and are found in centre of nucleus, replicates early, associates with transcription factories/hubs, associated with euchromatin. B compartments = inactive, late replicating, associated with nuclear lamina/ nucleolus, interacts less frequently, associated with heterocrhomatin. Nuclear structures/organising principles = lamina, nuclear pore, nucleolus, transcription factories, speckles (rna processing sites), polycomb bodies. Ways to probe nuclear architecture : hic, 3c, 4c, 5c = some have anchor primers, some don t, chia-pet,hi-chip, plac-seq, dam-id, 3d fish, oligo paint (fish) + live imaging (storm, orca) Chromatin domain boundaries = ctcf, cohesin, condensin, mediator. Lamin = proteins associated with nucleoplasmic side of inner nuclear membrane/ nuclear lamina. 3c, 4c, 5c, hic = methods to map loop formation, long range interations, chromosome conformation capture. Dam-id = dna adenine methyltransferase identification of binding heterocrhomatin. Chromatin compartments = sub chromosomal regions that interact with chromatin (a,b)
