BIOL 483 Lecture Notes - Lecture 85: Chromatin, Base Pair, Chromosome
Document Summary
Chromosomes don"t interact much with each other (proof by looking at chromosome territories to see where chromosomes occupy - label diff chromosomes with diff colours) > chromosomes are located in random territories/ neighbouring contacts are random. Chromosomes are reshuffled during cell cycle -> neighbouring contacts change during cell cycle. Most chromosome interactions occur in cis, but interacting with neighbouring chromosomes = difficult. Chromosomes territory = space that chromosome occupies, on range of nuclear scale. Within each chromosome territory there are compartments ( compartment = active region of chromatin ) B compartments = repressed region of chromosome, found near nuclear lamina, on range of chromosome scale. Within the b compartment, there are tads that are made up of sub-tads. > a and b compartments in each chromosome territory. > tads = example of long-range interaction mediated by ctcf protein, on range of megabase scale. > subtad = smaller units within tad, long-range interactions that hold chromatin tgt via enhancer-promoter interactions.
