BIO 101 Lecture Notes - Lecture 18: Missense Mutation, Reading Frame, Peptide
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Which of the following did Erwin Chargaff observe?
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The melting temperature (Tm) of a DNA duplex is:
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Given your knowledge and the descriptions of each of thesemethods, which of the following does NOT rely on the ability ofnucleic acids to hybridize with each other?
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Which of the following is a possible reason why DNA uses thymineinstead of uracil?
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Which of the following is an enzyme used in cloning to breakcovalent bonds?
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Which of the following could you use to remove the 5' phosphatesafter cleavage of a plasmid with a single type of restrictionenzyme to ensure that the plasmid would not simply fuse backtogether upon addition of ligase?
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Which of the following would you use to create a cDNA librarythat you would not use for a genomic library?
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A plasmid vector typically has which of the followingfeatures?
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Which of the following is FALSE of genomic or cDNAlibraries?
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Which of the following is NOT a typical component of apolymerase chain reaction (PCR)?
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How can a fusion protein be formed?
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Why is green fluorescent protein (GFP) so useful in visualizingfusion proteins in eukaryotes?
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You wish to determine the cellular location of a protein butunfortunately it is not particularly antigenic (can t develop astrong antibody response). How could you solve this problem?
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A positive result for the yeast two-hybrid analysis means thefollowing:
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Which of the following is the approximate size of the humangenome?
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Which of the following is correct about the structure orlocation of genes?
Question 22 options:
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1. A mutation in the gene encoding the integrase enzyme rendersthe protein non-functional. How would the affect the HIV infectioncycle?
Formation of the provirus would bestopped. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reverse transcription would nolonger be possible. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
The gp120 glycoprotein would nolonger be able to bind to the CCR5 receptor. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Cutting and assembling the capsid would be halted. 2. If the viral gene is AUGCCCAGG and the mRNA used to make theviral protein is UACGGGUCC, then the virus is a :
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