BIOL 303 Lecture 13: Genetics Week 13 Notes
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Department
BIOL - Biological Sciences
Course
BIOL 303
Professor
Waldman
Semester
Spring

Description
20.3 The Polymerase chain Reaction is a Power ni nique for Copying DNA start wl ennou cen of DNA amp Reverse ranscripticn PCR used to study gene express antitat e real-time PCR quantify amplificat han reo ctions as they occur 20.4 Molecular Techniques for Anolyzing DNA Restriction mano; est alousnes at B order of rest l Clicn es distance oet een sites agarose gel i th alum bromide Northern olo t analysis determine Whether a gene is being actively expressed given Cell or t S1ndy gene expression Cancer B genetic disorders Southern blot run a garost see parts of large genomic DNA smears Western same souinern at but use an own oody See pro 1 in 20 DNA Sequencing the mate Way to Characterize DNA St Mcivre av the Moe Level CWIar Sanger Coidcoxi seq Mencing use dideoxynvceGId and primer, Set up Sinines S r action, reaction stops her extension terminated when rom dideo ynuc cor de dATP) synthesized Chapter 21: nomics o informatics 8 Pro teom cs 21. vino e Genome snot una Sequencing a widely used mein for sequencing assemb en t re genome break genome into fragmen 3 COMEnde It and piece It together loy finding overlapping fragments using Softw aye as est way 10 SC quence Clone by approach DNA into small fragmens, clone mens, sequence Clones us frogmen s to p Wt clone oack together 20.3 The Polymerase chain Reaction is a Power ni nique for Copying DNA start wl ennou cen of DNA amp Reverse ranscripticn PCR used to study gene express antitat e real-time PCR quantify amplificat han reo ctions as they occur 20.4 Molecular Techniques for Anolyzing DNA Restriction mano; est alousnes at B order of rest l Clicn es distance oet een sites agarose gel i th alum bromide Northern olo t analysis determine Whether a gene is being actively expressed given Cell or t S1ndy gene expression Cancer B genetic disorders Southern blot run a garost see parts of large genomic DNA smears Western same souinern at but use an own oody See pro 1 in 20 DNA Sequencing the mate Way to Characterize DNA St Mcivre av the Moe Level CWIar Sanger Coidcoxi seq Mencing use dideoxynvceGId and primer, Set up Sinines S r action, reaction stops her extension terminated when rom dideo ynuc cor de dATP) synthesized Chapter 21: nomics o informatics 8 Pro teom cs 21. vino e Genome snot una Sequencing a widely used mein for sequencing assemb en t re genome break genome into fragmen 3 COMEnde It
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