BIO 226L Lecture Notes - Lecture 9: Lambda Phage, Dna Ligase, Tropism
Document Summary
11/04 - quiz 4 - lecture 9: bacteriophage biology. Most abundant organism on earth (~10 viruses per bacteria species) - >10 31. ~100 nm - ludicrously small (animal cell between 10-100 micrometer) Transduction - transfer of bacterial genetic material from a bacteriophage to a recipient cell. On an opaque lawn of bacteria spread the bacteriophage mixture clear-areas/plaques = where the phages are and they spread to nearby cells to make the circles (2-3 mm) Mix bacteriophage and cells at low multiplicity of infection (moi) - low number of phage particles with large number of cells. Each plaque = single infecting virus - single lineage. Head - ~50 nm - ~48,500 bps - double-stranded dna. 12 single-strand overhang with complementary base sequences on the ends = cos site. Ends annel and are joined by dna ligase in cells. Attachment to momp (maltose outer membrane porin - lamb) via. Penetration of 48. 5 dsdna linear genome via maltose permease channel.
