BIO 226L Lecture Notes - Lecture 1: Antonie Van Leeuwenhoek, Bouillon Cube, Amanita Muscaria
Document Summary
Anton van leeuwenhoek - lens maker made the first microscope (1600s) Today: 3d reconstruction, fluorescent probes, gram staining, other staining, etc. Organismal level - separation from a population to generate pure culture. Macromolecule level - (carbs, protein, nucleic acid, lipids) Central dogma of molecular biology: dna rna protein. Dna - heritable genetic material transcribed rna (serves as message translation into protein. Artificial media - nutrients (bouillon cube) + water. Autoclave (sterilization) - temp + pressure to create an environment in a chamber to kill all microorganisms. Except bacterial endospores (environmentally resistant eggs for bacterial cells) run cycle twice. Solid media petri dish = nutrients + water + agar (solidifying agent - derived from seaweed) stick to the plastic. Liquid media test tubes - erlenmeyer flask. Aseptic technique - to prevent contamination of cultures and media from microbes in the environment (transfer and contain microbes from one artificial media to another and keep them out of the environment)
