BIO 325 Lecture 5: Alternative splicing
• Alternative splicing
o DNA sequence information is not present in genomic library – alternative
splicing
o Different mRNAs can be produced by same transcript
o Rare transplicing events combine exons from different genes
• DNA sequence present in Genomic Library
o Promoter
o Introns
o Extrons
o 5’ untranslated region
Famous Experiments (1960s)
• The code is
o Collinear with amino acid sequence
o Non-overlapping = missense mutants
o A triplet = frameshift mutants, frameshift suppresoor mutants
o Decoding the triplets
• A gene’s nucleotide sequence – is a collinear the amino sequence of the encoded
polypeptide
• Co-linearity between DNA and amino acid sequence
o Each nucleotide is part of only a single codon
o Each point mutation altered only one amino acid
• 2 mutations resulting 2 different missense mutations can recombine because they are
physially separale
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