BIO 206L Lecture Notes - Lecture 6: Repeat Unit, Restriction Digest, Zygosity

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10 Apr 2017
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Analyze the gel to interpret success of a pcr reaction. Analyze the gel to interpret success of a restriction digest. Make observations of growth and phenotype of bacteria. Describe the relationship between genotype, the environment and phenotype. Mastermix: taqpolymerase, mgcl2, dna building- blocks,deoxyribonucleosidetriphosphates (dntps) Amplicon-copies of the one locus, product of pcr? each locus, two allels: one from mom and one from dad, can be homozygous or heterozygous. D1s80 repeat unit: 16 base pairs at that locus. How many repeats you have depend on how many you receive from your parents o range from 14-41 repeats: 369-801 base pairs, genotype example: 30, 14 there are 16 bp reapeat unit o. Gagga ccacc (g/a)g(c/a)aa g to prepare gel: melt agarose, 5 ul of syber safe o. Dna stain emits green light when under uv light: at 50 degrees c, pour into casting try, allow to cool and sloidify. Does not need to be in uv light.