BIOL 220 Lecture Notes - Lecture 5: Antiseptic, Lazzaro Spallanzani, Francesco Redi
Lecture 5
BIO 220
Control of Microbial Growth
Control of Microbial Growth
oPhysical violence
Heat
Radiation
filtration
ochemical violence
antibiotics
microbicides
Using Environmental Factors to Kill Microbes
oSterilization
Destroy all living cells, spores, and viruses on an object
oDisinfection
Kill or remove disease causing organisms on an object
oAntisepsis
Kill or remove disease causing organisms on living tissue
oSanitation
Decrease microbial levels to a safe level
Spontaneous Generation
oThe theory that living creature could arise without parents
oFrancesco Redi
Showed that maggots in decaying meat were the offspring of flies
oLazzaro Spallanzani
Showed that a sealed flask of meat broth sterilized by boiling failed to grow
microbes
oLouis Pasteur
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Document Summary
Using environmental factors to kill microbes: sterilization. Destroy all living cells, spores, and viruses on an object: disinfection. Kill or remove disease causing organisms on an object: antisepsis. Kill or remove disease causing organisms on living tissue: sanitation. Decrease microbial levels to a safe level. Spontaneous generation: the theory that living creature could arise without parents, francesco redi. Showed that maggots in decaying meat were the offspring of flies: lazzaro spallanzani. Showed that a sealed flask of meat broth sterilized by boiling failed to grow microbes: louis pasteur. Show that after boiling the content remain free of microbial growth despite the access to air. Once the flask was tipped and allowed the particles into the broth, microbial formation was observed: john tyndall. Boiled bacteria culture in an isolated flask and it showed signs of growth a few days without tipping which showed the sporulation.