BIOL 4320 Lecture Notes - Lecture 16: Microbiology, Citrobacter, Mycobacterium
16 Jun 2016
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Document Summary
16s rrna gene sequence analysis for identi cation of bacterial on clinical. In labs, shown that phylogenetic relationships of bacteria and all life-forms could be determined by comparing a stable part of the genetic code. Candidates for this genetic area: 5s, 16s (called small subunit), and 23s rrna and the spaces between these genes. Use of this gene sequence - seems to behave as a molecular chronometer, degree of conservation is assumed to result from the importance of the 16s rrna as a critical component of cell function. 16s rrna is also target for several antimicrobial agents. About 1550 bp long with variable and conserved regions: variable region is used for comparative taxonomy and the conserved for primers. Gene is universal in bacteria, so relationships can be measured among all bacteria. Gene allows for differentiation between organisms at the genus level across all major phyla of bacteria.
