MICROBIO 102 Lecture Notes - Lecture 10: Aerobic Organism, Coliform Bacteria, Gram-Positive Bacteria

• School: UW-Madison
• Department: Microbiology
• Course: MICROBIO 102
• Professor: All

Document Summary

Experiment 15 period 2 (15. 2) (water analysis general plate count and detection of coliforms) In period 2, we check the pca plates for the total aerobic plate count which as we learned in. Also in period 2, we check the lltb tube for growth and gas production. The medium is mildly selective against gram-positive bacteria, so growth (seen as cloudiness and/or sediment in the medium) is generally due to gram-negative bacteria. Among the populations of varioius kinds of organisms that are growing in the tubes, there may be organisms that can ferment lactose to acid and gas. a. There would have been no coliforms inoculated into and growing in any negative tube. ) Recall that last time we did not set up the coliform enrichment quantitatively as spelled out in the manual. We changed that to just inoculating one tube of lltb with one ml of the undiluted water sample.