BSCI-1510 Lecture Notes - Lecture 33: Cleavage And Polyadenylation Specificity Factor, Cytosol, Polypyrimidine Tract

• School: Vanderbilt University
• Department: Biological Sciences
• Course: BSCI-1510
• Professor: Singleton

Document Summary

5" splice site, 3" splice site, and branch point serve as recognition sequences: ---- aggu --------------------yyayyyy--------aggg-------- 5" splice site/junction: --- aggu ---- (--ag (cid:0) exon & Gu-- (cid:0) intron) branch site: ---- yyayyyy---- (polypyrimidine tract: y = pyrimidine (c, u) 3" prime splice site/ junction: ---aggg--- (--ag (cid:0) intron. & gg (cid:0) exon: snrnps bind to these sites, recruitment of spliceosomal proteins to assemble the spliceosome. & form a lariat of the intron-3"exon: 3" oh of freed 5"exon attacks phosphate at 3" splice junction to join the two eons & remove the intron. Steps of splicing: snrnps bind to 5" splice site, 3" splice site, & branch point recruits other splicing proteins (cid:0) functional spliceosome: within the spliceosome: the three regions are brought together in close proximity to join the axons together. Free intron (sometimes known as a lariat or lasso intron)