BSCI 2201L Lecture Notes - Lecture 12: Aedes Albopictus, Oocyte, Hematophagy

11 views4 pages
Musbah Shaheen
BSCI 202.2
Due 4/16/2015
Lab Report 12
Oogenesis
Abstract:
Oogenesis is a highly conserved process among vertebrates that leads to the production
of ova. In this lab session we studies the process of oogenesis in mosquitos by dissecting
Anopheles gambiae ovaries at multiple time points after the blood feeding tofollow egg
development.
Introduction:
Oogenesis is the process of forming an ovum from a primordial germ cell. Germ cells
migrate to the ovary during embryogenesis to form oogonia. Each oogonium goes through a
series of mitotic events to produce a primary oocyte. Primary oocytes arrest at the prophase of
the first meiotic division until sexual maturation, at which the primary oocyte completes Meiosis
1 to form a secondary oocyte and a polar body. Secondary oocytes complete Meiosis 2 to
produce mature ova and a second polar body. This process is usually accompanies fertilization.
The process of oogenesis is mostly conserved among vertebrates.
Insect reproductive tracks are characterized by having the spermatheca which stores
male sperm and produces nutrients to preserve the sperm. The process of oogenesis in Aedes
albopictus mosquito is contingent on hematophagy, the behavioral characteristic of feeding on
blood. In this lab session we observed the ovaries of Anopheles gambiae at multiple time points
after the blood feeding and follow egg development.
One of the consequences of blood feeding is that it makes mosquitos carriers for some
diseases like Malaria. There are five types of human malaria that are carried by 40 species of
Anopheles mosquitos. The malaria parasite enters the erythrocytes and causes their hemolysis.
In this lab session we examined infected erythrocytes at various stages of Plasmodium
development.
Because half of the world population is at risk of contracting malaria, it is important to
study the lifecycle of Anopheles mosquitos, in order to understand the transmission of malaria
and the effects it has on the body.
Materials and Methods:
Ovaries were isolated from Anopheles gambiae by using the dissecting microscope in
PBS. The samples were from different stages post blood meal were viewed under phase contrast
and fluorescence microscopy.
Prepared slides were used to view red blood cells at various levels of the Plasmodium
development. The prepared slides were viewed under phase contrast using the 40x oil objective.
Results and Conclusion
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