BIOS 2400 Lecture Notes - Lecture 4: Muscular Dystrophy, Creatine Kinase, Alkaline Phosphatase

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13 Mar 2017

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4. 1 enzymes as catalysts: enzymes: a class of proteins (exception ribozymes are made of rna) that serve as biological catalysts that. Induced-fit model: the enzyme undergoes a slight structural change to better fit the substrate; yields products: naming of enzymes, have the suffix -ase, the names of many enzymes specific both the substrate and the job category ex. Lactic acid dehydrogenase removes hydrogens from lactic acid. 4. 2 control of enzyme activity: measured by the rate at which substrate is converted to product. Influenced by: temperature, ph, concentration of cofactors and coenzymes, concentration of enzyme and substrate, possible stimulatory or inhibitory effects of products of enzyme function. Enzyme carbonic anhydrase: metabolic pathways: begins with an intial substrate, progresses through a number of intermediates, and ends with a final product error of. Albinism: dopa is a precursor for melanin; results from an inherited defect in the enzyme that catalyzes the formation of melanin from dopa: ex.

Related Questions

Explain how the following mechanisms regulate enzyme activity.

Part A

Covalent modification

a)The product of a series of reactions acts as an inhibitor for an earlier reaction.

b)Hormones control the synthesis of enzymes.

c)A regulator binds to the enzyme at a site other than the active site. This binding changes the shape of the enzyme and alters the catalytic ability of the enzyme.

d)An inhibitor binds reversibly to the enzymesubstrate complex, blocking the binding of the second substrate to the active site.

e)The activity of an enzyme is influenced by the addition or removal of a group that is covalently bonded to the enzyme.

f)An inhibitor forms covalent bonds to the active site, permanently blocking it.

Part B

Genetic control

a)The product of a series of reactions acts as an inhibitor for an earlier reaction.

b)Hormones control the synthesis of enzymes.

c)A regulator binds to the enzyme at a site other than the active site. This binding changes the shape of the enzyme and alters the catalytic ability of the enzyme.

d)An inhibitor binds reversibly to the enzymesubstrate complex, blocking the binding of the second substrate to the active site.

e)The activity of an enzyme is influenced by the addition or removal of a group that is covalently bonded to the enzyme.

f)An inhibitor forms covalent bonds to the active site, permanently blocking it

Part C

Allosteric regulation

a)The product of a series of reactions acts as an inhibitor for an earlier reaction.

b)Hormones control the synthesis of enzymes.

c)A regulator binds to the enzyme at a site other than the active site. This binding changes the shape of the enzyme and alters the catalytic ability of the enzyme.

d)An inhibitor binds reversibly to the enzymesubstrate complex, blocking the binding of the second substrate to the active site.

e)The activity of an enzyme is influenced by the addition or removal of a group that is covalently bonded to the enzyme.

f)An inhibitor forms covalent bonds to the active site, permanently blocking it.

Part D

Feedback inhibition

a)The product of a series of reactions acts as an inhibitor for an earlier reaction.

b)Hormones control the synthesis of enzymes.

c)A regulator binds to the enzyme at a site other than the active site. This binding changes the shape of the enzyme and alters the catalytic ability of the enzyme.

d)An inhibitor binds reversibly to the enzymesubstrate complex, blocking the binding of the second substrate to the active site.

e)The activity of an enzyme is influenced by the addition or removal of a group that is covalently bonded to the enzyme.

f)An inhibitor forms covalent bonds to the active site, permanently blocking it

Enzyme Activity

1. Briefly explain the principle of spectrophotometry.

2. Which of the following statements is true about enzymes?(check all correct answers)

		__ enzymes act on (are specific for) one or just a fewsubstrates
		__ enzymes can be denatured (loose their tertiary/quaternarystructure and activity) by extreme conditions such as heat, high orlow pH,or presence of organic solvents
		__most enzymes are DNA
		__ enzymes are protein
		__enzymes perform best at specific conditions of temperature, pHand salt concentration 5. Which enzymes break the following macromolecules? Match the following with their correponding answers: ___ Nucleic acids A. proteases or proteinases such as pepsin ___ Cellulose B. none ___ Triglycerides C. lipases ___ Proteins D. amylases ___ Starch E. nucleases 6.Match the following enzymes totheir products. Answers ___ pepsin, trypsin and other digestive proteases A.Nucleotides ___ salivary and pancreatic amylases and other enzymes B. AminoAcids ___ lipases C. Monosaccharides ___ nucleases D. fatty acids and glycerol 7. Based on experiments, what is the effect of extremeconditions of pH and temperature (boiling tube) on enzymeactivity? 8. ATP stores energy in the form of___ ___ Chemical energy ___ Kinetic energy ___ Thermal energy ___ Activation energy 9. An allosteric site in anenzyme is____ __ the site where reactions occur __ the site that binds aregulator __ the site that binds thesubstrate __ the site that binds the product 10. Enzymes increase the rate of reactions by making thetransition state less stable. __ True __ False 11. Enzymes increase the rate of reactions by placing reactingparts of the molecule in favorable positions. __ True __ False 12. Which of the followingis a mechanism for regulation of enzymes? __ covalent modification __ feedback inhibition __ allosteric regulation __ all of the above 13. Which of the following represents a correct order of eventsin an enzyme catalytic cycle? 1.enzyme changes shape 2.substrate binds to active site 3.substrate is converted to products 4. products are released from activesite __ 1>2>3>4 __ 2>1>3>4 __ 3>2>1>4 __ 2>4>3>1

cyancicada301

1. Compared to its energy content at rest, a vertebrate muscle that has just undergone heavy exercise would have:

a. more ATP and more ADP

b. less ATP and more ADP

c. more ATP and less ADP

d. less ATP and less ADP

2. When compared to a lung cell, a muscle cell would possess:

a. more mitochondria

b. more rough endoplasmic reticulum

c. less mitochondria

d. less rough endoplasmic reticulum

3.Enzymes are protein catalysts in organisms' bodies that permit them to carry out chemical reactions at relatively low temperatures. An enzyme is specific because only few kinds of substrate molecules fit in its activity site. Which of the following statements about enzymes is FALSE?

a. enzymes catalyze only a particular reaction of specific substrates

b. enzymes usually work only in a particular pH range

c. enzymes increase the energy of the reactant molecules

d. enzymes bind their substrates and hold them in a particular orientation

4. This process is essential if the two cells produced as a result of mitosis are to have a full complement of genes.

a. Replication c. Nondisjunction

b. Cytokinesis d. Synapsis

BIOS 2400 Lecture Notes - Lecture 4: Muscular Dystrophy, Creatine Kinase, Alkaline Phosphatase

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