CLS 1010 Lecture Notes - Lecture 2: Dioptre, Dicotyledon, Germination
Document Summary
Microscopy: eye completes the system as the focuser. Waves in phase are additive/brighter while out of phase are dim. Stereo-looking through microscope with two eyes concurrently: simplest form of light microscopy, transmitted white light illuminates sample from beneath, not much contrast, specimens appear transparent, staining needed, kills cells, stains will darken sample. Dic microscopy: differential interference, uses polarized light and specialized optics to produce image that appears 3d. Dark field: light waves directed from side, only scattered wave enter lenses, results in sample appearing white on dark/black background. Secondary markers with fluorescent molecule bind to antibody: green fluorescence protein. Fluoresces shows protein it was expressed w/ For clean up: turn off, unplug, wrap cord, lower stage, set to 4x objective, cover light. Bhk-21:fiberblast, involved in wound repair & actively dividing, isolated in 1961 by a. A431 cell line from 85 yro female squamous cell carcinoma, epithelial cells, found by dj. Plasmids designed for mammalian expression can be introduced via transfection.
