MCB 3020 Lecture Notes - Lecture 2: Pyruvic Acid, Conformational Change, Bactericide

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22 Jun 2021
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General Microbiology EXAM 2 Practice Questions
1. The non-transcribed region of DNA to which RNA polymerase binds to initiate transcription
is called the
A. promoter.
2. The Pribnow box of E. coli
A. is centered approximately 35 bp upstream of the start site of transcription
B. is centered between 7 and 13 bp upstream of the start codon
C. is centered approximately 10 bp upstream of the start site of transcription
D. is located at the site of addition of poly (A) in eukaryotic mRNAs
3. The genetic code is said to be __________ because more than one codon will specify a
particular amino acid.
A. ambiguous
B. degenerate
C. multiplicative
D. repetitious
4. The stop codons are translated codons; that is, they specify amino acids to be inserted into the
last position of a growing polypeptide chain.
True or False
5. In __________, ribosomes can attach to the mRNA and begin translation even though
transcription has not been completed.
A. prokaryotes
B. eukaryotes
C. fungi
D. protozoa and some plants
6. In the presence of both glucose and lactose, the lactose repressor is not bound to the operator
and the genes of the lactose operon are expressed.
True or False
7. Small RNAs that regulate translation by binding to the leader region of mRNAs are called
antisense RNAs.
True or False
8. __________ activate repressor proteins thereby decreasing the synthesis of certain enzymes.
A. Inducers
B. Corepressors
C. Effectors
D. Modulators
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9. __________ alter repressor proteins thereby increasing the rate of transcription initiation.
A. Inducers
B. Corepressors
C. Effectors
D. Modulators
10. The __________ is a factor-independent termination site found in the leader region of certain
operons, which, under the influence of ribosome behavior, controls the continued transcription of
that operon.
A. modulator
B. operator
C. attenuator
D. terminator
11. In __________ ribosomes can attach to the mRNA and begin translation even though
transcription has not been completed.
A. bacteria
B. humans
C. fungi
D. protozoa and some plants
12. Some microbial proteins are spliced after translation in a reaction that results in removal of
one or more internal intervening sequences called inteins.
True False
13. Semiconservative replication refers to
A. each base bonding at the 1' position of the sugar.
B. a purine always bonding to a pyrimidine.
C. one helix strand that runs from the 5' to 3' direction and the other strand that runs from the 3'
to 5' direction.
D. an original parent DNA strand and one newly synthesized DNA strand comprising a
new DNA molecule.
14. Very few gram negative bacteria and other soil bacteria utilize the Entner-doudoroff
glycolytic pathway False
15. Which of the following processes generates the greatest amount of energy?
A. anaerobic respiration
B. Aerobic respiration (encompasses C, D, E)
C. Krebs Cycle
D. Glycolysis
E. Oxidative phosphorylation
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Document Summary

True or false: small rnas that regulate translation by binding to the leader region of mrnas are called antisense rnas. True false: at 4:00 p. m. a closed flask of sterile broth is inoculated with 10,000 cells. At 9:00 p. m. the culture enters stationary phase with a population of 65 million cells. __________ phosphorylation: chemiosmotic, oxidative, substrate-level, conformational change, the most common pathway for conversion of glucose to pyruvate is, entner-doudoroff, pentose phosphate, embden-meyerhoff, mixed acid fermentation, the limitation on microbial growth rate at high nutrient levels is. A. lag b. exponential c. stationary d. decline: when a young, vigorously growing culture of bacteria is transferred to fresh medium of the same composition, t lag phase is usually short or absent. At 9:00 p. m. the log phase culture has population of 65 million cells. The approximate number of generations that has occurred is b. 13: at 4:00 p. m. a closed flask of sterile broth is inoculated with 10,000 cells.

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