2. Solve the following DE using an Substitution:

(X³ +y³) dx+ 3xy² dy=0, y(0)=4

MsbA is an ABC transporter that transports LPS from the cytoplasmic to periplasmic leaflet of the inner membrane of E. coli (flipase).

A) Diagram the basic mechanism how this ABC transporter would transport LPS across the membrane.

B) You have isolated a variant of MsbA that has the Q-loop mutated from a Q to a V. How do you think this will affect the function of MsbA?
Design a set of experiments to test your hypothesis and explain what you expect to observe.

The secondary active transporters LacY, Adenine nucleotide translocase, and Phosphate translocase all utilize the gradients created by the ETC to drive the transport of their solutes. Assuming the rate of translocation for each transporter is the same, which transporter is depleting the ETC electrochemical energy gradient the fastest?

Design an experiment to test your hypothesis and explain what you expect to observe in your experiment

Which detergent would be best suited for the membrane extraction or refolding for each of the integral membrane proteins in
the following list? For each of the integral membrane proteins listed, would you choose to extract or refold and why?
1. LamB
2. Complex I (ETC)
3. LacY
4. OmpC
5. ABC transporter
6. GPCR      

Herald Golf, Inc., was formed in September of last year. The company designs, contracts for the manufacture of, and markets a line of men’s golf apparel. A portion of the statement of cash flows for Herald follows:

Management expects a solid increase in sales in the near future. To support the increase in sales, it plans to add $2.2 million to inventory. The company did not disclose a sales forecast. At the end of the current year, Herald had less than $1,000 in cash. It is not unusual for a new company to experience a loss and negative cash flows during its start-up phase.

Required:

As a financial analyst recently hired by a major investment bank, you have been asked to write a short memo to your supervisor evaluating the problems facing Herald. Emphasize typical sources of financing that may or may not be available to support the expansion.

What is time?

How come first egg or hen?

Why rainbow s form ?

What is rainbow?

What is laptop?

What is Love triangle?

What is reproduction?

3. Write a short analysis of the food plate. Answer the following in your analysis:

a) What is the food plate?

b) Why do you think the government created this tool?

c) After investigating the website do you think the tools are useful? Why or why not?

d) How could you use this information? Provide examples of how and why you would use this information for one full day.