🏷️ LIMITED TIME OFFER: GET 20% OFF GRADE+ YEARLY SUBSCRIPTION →
Log in
Biology
18
answers
0
watching
502
views
1 Jan 2018

In SSP-PCR, why is it important to determine genomic DNA concentration and quality for interpretation of the test results?

Watch
18
answers
0
watching
502
views

For unlimited access to Homework Help, a Homework+ subscription is required.

Unlock all answers

Get 1 free homework help answer.
Already have an account? Log in
Share
Already have an account? Log in
Share
Already have an account? Log in
Share
Already have an account? Log in
Share
Already have an account? Log in
Share
Already have an account? Log in
Share
Already have an account? Log in
Share
Already have an account? Log in
Share
Already have an account? Log in
Share
Already have an account? Log in
Share
Already have an account? Log in
Share
Already have an account? Log in
Share
Already have an account? Log in
Share
Already have an account? Log in
Share
Already have an account? Log in
Share
Already have an account? Log in
Share
Already have an account? Log in
Share
Bunny Greenfelder
Bunny GreenfelderLv2
2 Jan 2018
Already have an account? Log in
Share

Related textbook solutions

Molecular Cell Biology
8 Edition,
Lodish
ISBN: 9781464183393
Biology: Science for Life with Physiology
6 Edition,
Belk
ISBN: 9780134555430
Concepts of Biology
OER Edition,
Openstax
ISBN: 9781938168116
Biology
2nd Edition, 2013
Openstax
ISBN: 9781947172517
Essentials of Biology
5 Edition,
Mader
ISBN: 9781259660269

Related questions

So I performed an experiment using my own human genomic DNA andPCR. The experiment did not work.

Could someone give me a detailed explanation as to why the PCRdidn''t work?

***Some details***

Isolated Human genomic DNA in water, Human derived PCR productsand Restriction Enzyme Digestion of Human derived PCR products werethe 3 types of DNA loaded onto the agarose gel.

A research associate working for Intragene Therapeutics prepares an investigative PCR reaction to screen human tissue samples for specific mutations. He uses 55 ng of genomic DNA from one tissue sample in a single PCR reaction, to amplify a 1,246 bp fragment. The PCR protocol requires a final concentration for two primers of 1.5 uM each, and a final concentration of 180 uM for dNTPs, in a total PCR reaction volume of 65 uL. During the PCR process, the fragment is amplified for 33 cycles. He quantifies the amount of PCR products to be 74 ng/uL.

1. If his purified genomic DNA template stock has a concentration of 1.53 mg/mL, and he dilutes it 1:100, how many microliters of this dilution does he use? Answer to one decimal place.

2. He decides to dilute his DNA template stock so that he can pipet a 15 uL volume that contains 55 ng of genomic DNA into his PCR reaction. If he starts with 1 uL of the DNA template stock, what is the total volume of the dilution he needs to make? Answer to the nearest whole number.

3. How many copies of the fragment are produced in his PCR reaction? Assume that the target sequence is present in only one copy in the genome, as in a haploid human genome. Answer to the nearest whole number.

4. What is the amount of amplification of the PCR product in his reaction - that is, the fold increase in the amount of amplified DNA segment? Answer in decimal notation.

5. If one of the primer stocks in his lab has a concentration of 50 uM, what volume (in microliters) does he add of this primer to have the final concentration specified in the PCR protocol? Answer to the nearest whole number. 6. The dNTP master mix available in the company lab has a concentration of 12 mM total dNTP. What volume, in microliters, of this master mix does he add to his PCR reaction to have the final concentration specified in the PCR protocol? Answer to the nearest whole number.

Answers. 1. 3.6 uL 2. 417 uL 3. 16,667 copies 4. 210,000,000 5. 2 uL 6. 1 uL

A research associate working for Intragene Therapeutics prepares an investigative PCR reaction to screen human tissue samples for specific mutations. He uses 55 ng of genomic DNA from one tissue sample in a single PCR reaction, to amplify a 1,246 bp fragment. The PCR protocol requires a final concentration for two primers of 1.5 uM each, and a final concentration of 180 uM for dNTPs, in a total PCR reaction volume of 65 uL. During the PCR process, the fragment is amplified for 33 cycles. He quantifies the amount of PCR products to be 74 ng/uL.

2. He decides to dilute his DNA template stock so that he can pipet a 15 uL volume that contains 55 ng of genomic DNA into his PCR reaction. If he starts with 1 uL of the DNA template stock, what is the total volume of the dilution he needs to make? Answer to the nearest whole number.

3. How many copies of the fragment are produced in his PCR reaction? Assume that the target sequence is present in only one copy in the genome, as in a haploid human genome. Answer to the nearest whole number.

4. What is the amount of amplification of the PCR product in his reaction - that is, the fold increase in the amount of amplified DNA segment? Answer in decimal notation.

Related Documents

BIO 361 Lecture Notes - Lecture 12: Rheumatoid Arthritis, Hydrolysis, Skeletal Muscle
byzantiumtortoise920
BIO120H1
Final Exam
Study Guide
BIO120H1 Study Guide - Quiz Guide: Glucose Oxidase, Blood Sugar, Spectrophotometry
sangriaalligator793
BIO200H5 Lecture Notes - Lecture 7: Frequency Distribution, Drug Interaction, Drug Tolerance
pinkhyena692

Weekly leaderboard

Home
Homework Help 3,900,000
Biology 460,000
Start filling in the gaps now
Log in