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7 Mar 2018

METHYLATION

1-What is methylation and where does it usually occur? What effect does it have on gene expression?

2-What is the difference between de novo methylation and maintenance methylation?

3-What is epigenetics, and what is genomic imprinting?

4-Where are promoters usually found on chromatin?

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Beverley Smith
Beverley SmithLv2
7 Mar 2018

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Related questions

Chapter 10

1.Outline the history of our knowledge on DNA up to Watson and Crick. What were the main contributions made by each researcher’s key experiment?

2.Explain the setup of the Hershey and Chase experiment, what would the results have been if protein was the genetic material?

3.Draw the structure of a DNA nucleotide, labeling each main component correctly. How does an RNA nucleotide differ?

4.If a section of double stranded DNA contains 19% Adenine, how much Thymine is present?

5.You are a researcher studying the genetic basis of heart attacks and have been working to determine the expression levels of different genes that might contribute to cancer formation. You obtain the DNA methylation status of five genes of interest (the data are shown in the table below). The plus (+) sign indicates the level of DNA methylation; more plus signs correlates with increased methylation levels.Based on this information which genes would you predict to have the highest rate of transcription?

Gene

Methylation levels

1

++

2

+++++

3

+++

4

++

5

+

What are the characteristics of the 3 main DNA forms?

Chapter 11

What are the different types of chromatin?

What are the structures and important roles for telomeres and centromeres?

What are the differences found between eukaryotic chromosomes and mitochondrial?

Chapter 12

Explain each of the different models of replication.

If you grow a culture of bacteria in media with radioactive nucleotides so that all DNA in the cells include radioactive nucleotides and then place the bacteria in new non radioactive media. After two rounds of replication what proportion of the DNA molecules will contain radioactivity?

Summarize the similarities and differences between rolling-circle replication, theta replication and linear eukaryotic replication.

What are the functions of the different DNA polymerases found in eukaryotic cells?

Draw a replication fork and include all key components and orientations. (Leading/lagging strands, DNA helicase, RNA primer and DNA gyrase)

What is the Holliday model of recombination and what are the necessary steps?

Chapter 13

What are the different types of RNA and what roles do they play?

Describe the properties and functions of each of the RNA polymerases and how they differ depending on the organism.

Describe in detail the process and mechanisms of transcription in both prokaryotes and eukaryotes.

Chapter 14

What are the primary purposes of each of the three post transcriptional modifications that occur in eukaryotic cells.

What is alternative splicing and what role does it play in the cell?

How is ribosomal RNA processed after transcription?

How do siRNA and miRNA work, describe/draw out the process in detail.

I am reviewing this article for a biochemistry presentation and need help understanding it. http://www.jbc.org/content/289/27/18707.full?sid=dd97d7e5-e294-4704-bcd6-b2bef3f37b34#cited-by

My question is what background information is going to be necessarry/important for my audience (fellow biochemistry students) to understand what I'm talking about? So far I've explained that epigenetics is the study of changes in gene expression (no change in DNA sequence from these changes), THC acts on CB1 and CB2 receptors which are GPCRs, and CB2 receptors are found on immune cells. I also plan on including that methylation generally results in genes not being expressed and that THC causes Th1 cytokine genes to be suppressed and Th2 genes to be expressed, resulting in anti-inflammatory effects. Please correct me if this is wrong. I also plan to explain what cytokines and histones are. Anything else you may think would be helpful is greatly appreciated. Thank you

1:What is the principle ofsegregation? Why is it important?

2:What is the principle ofindependent assortment? How is it related to theprinciple

of segregation

3:How are Mendel%u2019sprinciples different from the concept of blendinginheritance?

4:What is the concept of dominance? How does dominance differfrom incomplete

dominance

5:What is the chromosometheory of inheritance? Why was it important?

6:Distinguish among the following terms: locus, allele,genotype, and phenotype.

7:How did Mendel know that each of his pea plants carried twoalleles encoding a

characteristic?

8:How do incompletedominance and codominance differ?

9:What is incompletepenetrance and what causes it

10:What is geneinteraction? What is the difference between an epistatic gene anda

hypostaticgene?

11:What is a recessiveepistatic gene?

12:What characteristics are exhibited by a cytoplasmicallyinherited trait?

13:What is genomic imprinting?

14:What is the differencebetween genetic maternal effect and genomic imprinting?

15:What is the difference between a sex-influenced gene and agene that exhibits

genomicimprinting

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