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Design an experimentin which you will test the effect of an acidic fluid on enzymaticactivity. (Recall: enzymes are proteins.) To complete this project,it may be useful for you to review the Scientific Method Tutorial (found the CourseContent section of the classroom under the Science Learning Centerlink) and the Scientific Method lab (Lab 1), so that you canbetter understand how to design an experiment. It may also behelpful for you to review Lab 4 (Enzymes). As you review Lab 4, youwill be reminded that there are several factors that impactenzymatic activity: pH, temperature, and amount of reagent. Feelfree to refer to observations and information from Lab 4 as youcomplete the questions required in the Final Applied Project (seethe questions below).

As you design yourexperiment for this project, please remember that you are trying toexamine how an acidic fluid will modify the outcome of an enzymaticreaction. To successfully complete this project, you will need toidentify the question(s) being asked in your experiment and thehypothesis that you are testing. In your experimental design, youmust clearly explain what you are doing. That means that you willneed to identify the enzyme and the acid, as well as explain yourexperimental protocol (this information will help you to answerquestion 2). You must alsothoroughly explain how the addition of the acidic fluid impactedthe overall reaction process (this information will help you toanswer question 4).

Hint: Keepin mind that the acid will change the environmental conditions ofthe experiment (for example, a low pH value could change the shapeof the active site on the enzyme protein), without directlyparticipating in the reaction.

please address the following:

1. What question are you asking?Clearly state your hypothesis.

2. Design an experiment. Provide adetailed account of the materials and methods used to conduct theexperiment. Also include the methods for data collection andanalysis.

3. Conduct the experiment and recordyour results below. What did you observe? Which samples showedbubbling?

4. Use your knowledge about factorsthat effect enzymatic activity to interpret your results. It may benecessary for you to refer to your lab manual and/or otherresources. What effect did the acid have on the enzyme?

5. Was your hypothesis supported? Whatis your conclusion? Explain your answer.

6. Cite all references used tocomplete your Final Applied Project.


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Elin Hessel
Elin HesselLv2
29 Sep 2019
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