PoB researcher Zhongshan has 10 yeast mutants that he isolated by mutangenizing yeast. None of them can grow on minimal media alone, or on minimal media (MM) supplemented with ornithine (Orn). He also finds that all of these mutants can grow on citrulline (Cit) or arginine (Arg). He wants to check if these mutants are "allelic", (i.e. the hypothesis that they affect the same gene), or if any are non-allelic (i.e. the hypothesis that they affect different genes). Which of the following would provide the best test of these alternative hypotheses?
Test if the mutants can grow when both Cit and Orn are added to MM.
Test if the mutants synthesize Arg or not when grown in the presence of Orn.
Do pairwise crosses of the mutants to see if their diploid progeny (i.e. daughter cells with a copy of every gene from one mutant parent plus a copy of every gene from the other mutant parent) can grow on MM supplemented with Cit or not.
Do pairwise crosses of the mutants to see if their diploid progeny can grow on MM supplemented with Orn or not.
None of the above experiments will be able to distinguish between allelism or non-allelism.
PoB researcher Zhongshan has 10 yeast mutants that he isolated by mutangenizing yeast. None of them can grow on minimal media alone, or on minimal media (MM) supplemented with ornithine (Orn). He also finds that all of these mutants can grow on citrulline (Cit) or arginine (Arg). He wants to check if these mutants are "allelic", (i.e. the hypothesis that they affect the same gene), or if any are non-allelic (i.e. the hypothesis that they affect different genes). Which of the following would provide the best test of these alternative hypotheses?
Test if the mutants can grow when both Cit and Orn are added to MM.
Test if the mutants synthesize Arg or not when grown in the presence of Orn.
Do pairwise crosses of the mutants to see if their diploid progeny (i.e. daughter cells with a copy of every gene from one mutant parent plus a copy of every gene from the other mutant parent) can grow on MM supplemented with Cit or not.
Do pairwise crosses of the mutants to see if their diploid progeny can grow on MM supplemented with Orn or not.
None of the above experiments will be able to distinguish between allelism or non-allelism.
