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skytortoise751Lv1

28 Sep 2019

1- Describe the stages of C. elegans development. How many
stages were you able to identify?

2. Why is it necessary for C. elegans to pass through several
larval stages, and how is this type of development different
from humans?

3. How does a hermaphrodite produce offspring without mating?

4. What physical (morphological) differences did the mutant worm?What differences in behavior or movement? did you identify thesame
characteristics of the mutant C. elegans?

5. For each mutant phenotype , what do you think
would be the function of the protein produced by thewild-type
gene?

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Nelly StrackeLv2

28 Sep 2019

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In a genetic experiment:

The use and limits of RNA interference as a reverse genetics technique for knocking down gene expression were tested. The wild-type and mutants worms for the strains were selected. The RNAi feeding strains and control bacterial were used for the the RNAi experiment. The eri-1 C. elegan strains (wild-type) were used for RNAi treatment and mutant strains that were used ( unc-22 and dpy-13) C. elegans. Both carbencillin and IPTG wered added to the RNAi feeding plates and E.coli containing the RNAi transgenes were spread on the plates. The antibodic carbacillin only allows growth of bacteria which contains the RNAI construct. IPTG induces the expression of the polymerase which then transcribes the siRNA in the bacteria. 6 NGM plates without bacteria were used and 20ul of 12.5 mg/ml carbencillin concentration were spread on the 6 of the plates. The carbencillin dried and then IPTG was spread (25ug/ml) on each plate. Then 6 plates with the RNAi bacterial strains were used. Theree of plates for strain one and the other three for strain the second strain. When IPTG dried , 150 ul of the appropraite bacteria was added onto each plate. 2NGM plates (without carbenicillin or IPTG) was spread with 150 ul of OP50 bacterial culture. OP50 is the regular E. coli strain for culturing C. elegans and does not contain the RNAi constructs. These plate were used as the control plates6. Then the plates were incubated for two days. Then the adult hermaphrodites from the RNAi feeding plates and the op50 plates were removed. Then from the eri-1 wildtype stock, 2 or 3 L4 hermaphrodites or
young adults were transferred onto each RNAi feeding plate. Then L4 worms were transferred to an intermediate plate first. After they have a minute to move away from each other and the eggs, and transfered to the RNAi plates. On the two control plates seeded with OP50, 2 L4 or young adult hermaphrodites were placed there. Plates were obtain as the reference mutants (unc-22, bli-1 or unc-13). Then the plates were incubated to allow the worm to develop and lay eggs in two days. Then the adult hermaphrodites from your RNAi feeding plates and the op50 plates were removed and then placed back into the incubator.

The op500 was the negative control plate wtih 80 and 300 progeny and unc-22 had around 50. (reference plates)

Mating in the RNAi plates

Dpy (60) x wildtype (15) and unc( 70) x wildtype ( 8) progeny

the phenotype was movement and it seemed similar phenotype within the progeny.

Can you help me understand the results and what is happening genetically in the experiment ? Are the for dyp-15 x unc-22 mating cross and unc-22 x eri-1 wildtype C. elegans. Based on the data:

Briefly discuss the results of the RNAi experiment. How closely did the RNAi-treated worms phenocopy the mutant worms?

Was the gene knockdown 100% effective?

Was the RNAi treatmentequally effective for the two genes? If not, discuss this result and why that could occured.

Please be specific and detailed

Genetics HELP ASAP

Genetics HELP ASAP In a genetic experiment: The use and limits of RNA interference as a reverse genetics technique for knocking down gene expression were tested. The wild-type and mutants worms for the strains were selected. The RNAi feeding strains and control bacterial were used for the the RNAi experiment. The eri-1 C. elegan strains (wild-type) were used for RNAi treatment and mutant strains that were used ( unc-22 and dpy-13) C. elegans. Both carbencillin and IPTG wered added to the RNAi feeding plates and E.coli containing the RNAi transgenes were spread on the plates. The antibodic carbacillin only allows growth of bacteria which contains the RNAI construct. IPTG induces the expression of the polymerase which then transcribes the siRNA in the bacteria. 6 NGM plates without bacteria were used and 20ul of 12.5 mg/ml carbencillin concentration were spread on the 6 of the plates. The carbencillin dried and then IPTG was spread (25ug/ml) on each plate. Then 6 plates with the RNAi bacterial strains were used. Theree of plates for strain one and the other three for strain the second strain. When IPTG dried , 150 ul of the appropraite bacteria was added onto each plate. 2NGM plates (without carbenicillin or IPTG) was spread with 150 ul of OP50 bacterial culture. OP50 is the regular E. coli strain for culturing C. elegans and does not contain the RNAi constructs. These plate were used as the control plates6. Then the plates were incubated for two days. Then the adult hermaphrodites from the RNAi feeding plates and the op50 plates were removed. Then from the eri-1 wildtype stock, 2 or 3 L4 hermaphrodites or young adults were transferred onto each RNAi feeding plate. Then L4 worms were transferred to an intermediate plate first. After they have a minute to move away from each other and the eggs, and transfered to the RNAi plates. On the two control plates seeded with OP50, 2 L4 or young adult hermaphrodites were placed there. Plates were obtain as the reference mutants (unc-22, bli-1 or unc-13). Then the plates were incubated to allow the worm to develop and lay eggs in two days. Then the adult hermaphrodites from your RNAi feeding plates and the op50 plates were removed and then placed back into the incubator. The op500 was the negative control plate wtih 80 and 300 progeny and unc-22 had around 50. (reference plates) Mating in the RNAi plates Dpy (60) x wildtype (15) and unc( 70) x wildtype ( 8) progeny the phenotype was movement and it seemed similar phenotype within the progeny. Can you help me understand the results and what is happening genetically in the experiment ? Are the for dyp-15 x unc-22 mating cross and unc-22 x eri-1 wildtype C. elegans. Based on the data: Briefly discuss the results of the RNAi experiment. How closely did the RNAi-treated worms phenocopy the mutant worms? Was the gene knockdown 100% effective? Was the RNAi treatmentequally effective for the two genes? If not, discuss this result and why that could occured. Please be specific and detailed

Cells produced in the incision-healing process in the catâs abdomen would be ____ the other cells on her abdomen.

a) half the size of

b) haploid versions of

c) genetically different from

d) genetically identical to

During the same visit, your veterinarian recommended that your cat also receive a vaccination for feline distemper. This vaccine stimulated the catâs immune system to produce which one or more of the following?

a) antigens b) memory cells c) antibodies d) antibiotics e) both b & c

[1] The male elephant seal (l) is much larger than the female (r) of the species. Males spend very little time raising their young. You would correctly predict that the maleâs size is due primarily to _________ and that he is ___________in his mating habits.

a) natural selection; monogamous

b) sexual selection; polygamous

c) natural selection; polygamous

d) sexual selection; monogamous

[2] The Good Genes Hypothesis would account for which of the following characteristics of males that attract the most females?

a) Peacock tails, which prevent males from seeing behind them when they court

b) Sailfin molly, a dorsal-finned fish, which are enormous and easily spotted by predators

c) Guppy tailfins, which reduce their swimming speed.

d) Gray tree frogs, that call the longest and produce offspring that mature quickest

e) Fish that produce bright colors, yet have very weak immune systems.

[3] In mice, a recessive autosomal allele (o) results in obesity because the mice are unable to produce the hormone leptin from their fat cells.Normal mice with the dominant allele (O) do produce leptin. Homozygous recessive (oo) obese mice injected with leptin are observed to lose weight.

In a cross between two mice that are heterozygous for the obesity gene (Oo), you would predict which one of the following?

a) All the offspring will be obese.

b) All the offspring will be normal.

c) 50% of offspring will be able to produce leptin.

d) Obese offspring would eat less and burn more calories if injected with leptin.

e) Fat cells in obese mice release more leptin than those of their normal parents.

[4] A scientist discovers a new mutant mouse that he calls âChunkyâ because they are 80% heavier than normal mice. Which one or more of the following predictions would be correct?

a) Chunky mice could have the âddâ genotype.

b) Normal mice produce more thyroxin (thyroid hormone) than Chunky mice.

c) Glycolysis would occur more rapidly in Chunky mice than normal mice.

d) A protein that blocks leptin receptors would promote weight loss in all Chunky mice.

e) Both (a) & (b) are correct.

[5] When she was 6 months old, your cat was surgically altered so that they could not reproduce. This required a small incision into the catâs abdomen to remove the ovaries. This incision healed by a type of cell division called ______.

a) endocytosis b) meiosis c) mitosis d) exocytosis e) fusion

[6] Cells produced in the incision-healing process in the catâs abdomen would be ____ the other cells on her abdomen.

a) half the size of

b) haploid versions of

c) genetically different from

d) genetically identical to

[7] During the same visit, your veterinarian recommended that your cat also receive a vaccination for feline distemper. This vaccine stimulated the catâs immune system to produce which one or more of the following?

a) antigens b) memory cells c) antibodies d) antibiotics e) both b & c

How did the imagery of witches flying upon broomsticks originate? Historians have found evidence that some medieval European âdrug cultsâ included women who would prepare a potion by grinding up belladonna plants into an extract. Known as an anesthetic and muscle relaxant, this extract was applied using long sticks on the skin for rapid absorption into the bloodstream. The resulting symptoms included slowed breathing, dilated pupils, heavy eyelids, and a delirious sensation of flying.

[8] If one of these âwitchesâ wanted to counteract her shallow breathing and muscular weakness, you might recommend that she use â¦

a) botulinum toxin--an inhibitor of the release of acetylcholine.

b) calabar beans that contain an inhibitor of acetylcholinesterase.

c) a frog toxin that keeps the sodium ion (Na+) channels of the axon stuck opened.

d) curare, which blocks acetylcholine binding to post-synaptic muscle cells.

e) oubain, a plant toxin which inhibits the Na+/ K+ATPase pump.

After running around, a dog lies down to rest. Which of the following does not describe the flow of heat?

a. The dog loses heat by conduction to the warmer ground.
b. The dog loses heat by the evaporation through panting.
c. The dog loses heat by convection to the wind.
d. The dog gains heat by the radiation from the sun.
e. The dog generates heat by respiration.

What is the smallest number of DNA nucleotide sequence changes that must occur to cause a change in one amino acid in a particular protein?

a) 0 b) 1 c) 2 d) 3 e) 64