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  1. Besides ending the controversy over spontaneous generation, what other accomplishments do we credit to pasteur? 

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Related questions

As we all should know, abiogenesis and spontaneous generationare far from identical. For one, spontaneous generation wasdisproven with Pasteur, and abiogenesis seems to be a solidscientific theory. However, one can loosely summarize both as "theemergence of life from non-living materials" which, at the veryleast, is confusing for the layman.

My question is: What truly differentiates abiogenesisfrom spontaneous generation? For example, if abiogenesiswere to happen again, how would we differentiate the two terms?

Is time the most contributing factor (abiogenesishappened over hundreds of millions of years)? Or the fact thatabiogenesis generates "primitive organisms", while spontaneousgeneration assumes the generation of evolved, complexorganisms?

Identify the independent variable.

Prior to the mid-1800s, people believed that bacteria appearedin the food through spontaneous generation, which held that livingorganisms arose spontaneously from non-living matter (such asdust). In 1862, Louis Pasteur questioned whether spontaneousgeneration was possible. He boiled several batches of broth (growthmedium) to kill any existing bacteria. After boiling, all batchesof broth were then exposed to the air, but some of the boiledbatches of broth were protected by filters or long, twisting tubesto prevent dust and other particles from passing through to thebroth. His results showed that a variety of organisms grew in thebroths that were not protected by filters and tubes, but nothinggrew in the broths that were protected by filters or tubes. Basedon this description, what is the independentvariable?

1) Whether there is any growth of microorganisms.

2) How long the broth is boiled.

3) How long the broth was exposed to air.

4) Spontaneous generation.

5) Whether or not the broth was exposed to air.

I was thinking the independent variable had something to do withwhether the broth was protected by filters/twisty tubes or not.

What other tools do researchers use to observe cell behavior besides GFP?

What similarities do they share with GFP?

What are the differences?

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