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22 Aug 2018
A graduate student wants to compare the genome-wide gene expression patterns in normal cells and breast cancer cells. His advisor told him to purify mRNAs from both samples, convert mRNAs to cDNAs, use parallel-sequencing method (e.g. the pyro-sequencing talked in the class) to sequence the resulting cDNA, and then count the copy numbers of cDNA in each sample. This pipeline of experiments is termed as
cDNA sequencing
RNA sequencing
microarray
hybridization
A graduate student wants to compare the genome-wide gene expression patterns in normal cells and breast cancer cells. His advisor told him to purify mRNAs from both samples, convert mRNAs to cDNAs, use parallel-sequencing method (e.g. the pyro-sequencing talked in the class) to sequence the resulting cDNA, and then count the copy numbers of cDNA in each sample. This pipeline of experiments is termed as
| cDNA sequencing | ||
| RNA sequencing | ||
| microarray | ||
| hybridization |
Deanna HettingerLv2
25 Aug 2018
