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11 Dec 2019
Part A)
From the information below, determine how many micromoles of alkaline phosphatase are in the reaction tubes.
The molecular weight of alkaline phosphatase is 140 kDa
The activity of the enzyme sample is 1783 units/mg protein
Protein concentration of stock enzyme is 19.64mg/ml
Stock enzyme was diluted to a working concentration of 1 ul/10ml Buffer
The assay tubes contain 20 ul of enzyme in a reaction volume of 3 ml
Part B) The turn over number is the number of moles of product produces by one mol of enzyme at Vmax. Using the results from above and the Vmx from the linear graph, calculate the turn over nummber for alkaline phosphatase in this exercise. Given that Vmax is 50.
Part A)
From the information below, determine how many micromoles of alkaline phosphatase are in the reaction tubes.
The molecular weight of alkaline phosphatase is 140 kDa
The activity of the enzyme sample is 1783 units/mg protein
Protein concentration of stock enzyme is 19.64mg/ml
Stock enzyme was diluted to a working concentration of 1 ul/10ml Buffer
The assay tubes contain 20 ul of enzyme in a reaction volume of 3 ml
Part B) The turn over number is the number of moles of product produces by one mol of enzyme at Vmax. Using the results from above and the Vmx from the linear graph, calculate the turn over nummber for alkaline phosphatase in this exercise. Given that Vmax is 50.
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