True or False.
The SIMS test is often used to look for motility. ______
The Simmons Citrate test is looking for acid production from the bacteria. _______
MSA plates grow only gram â bacteria. _______
E. Coli produces an orange colony on EMB plates, due to lactose fermentation. _______
Gas production in TSI test is often seen as cracks in the media. _______
The LIA tests changes from green to blue when positive. _______
Klebsiella pneumoniae produces a black butt on a TSI test. _______
Salmonella typhimurium is gram-positive bacteria. ________
The MR-VP media was inoculated using the slant method. _______
If bacteria grow only on the butt of the biochemical test, then it is considered aerobic.
True or False.
The SIMS test is often used to look for motility. ______
The Simmons Citrate test is looking for acid production from the bacteria. _______
MSA plates grow only gram â bacteria. _______
E. Coli produces an orange colony on EMB plates, due to lactose fermentation. _______
Gas production in TSI test is often seen as cracks in the media. _______
The LIA tests changes from green to blue when positive. _______
Klebsiella pneumoniae produces a black butt on a TSI test. _______
Salmonella typhimurium is gram-positive bacteria. ________
The MR-VP media was inoculated using the slant method. _______
If bacteria grow only on the butt of the biochemical test, then it is considered aerobic.
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microbiology double unknown
1. G+ coccus
*tiny colonies
*white colony
*sediment
*Phenol Red:
PR Glu: (A/-) Yellow
PR Lac: (A/-) Yellow
PR Suc: (A/-) Yellow
*SIM test
Sulfure reduction: (-) No black
Indol: (-) No pink
Motility: (+)
*MR: (+) Red
*VP: (+) Red (redish brown)
* Red blood agar: beta hemo
*MacConkey: (-) No growth (G+)
*DNAse: (+) clearing around
*Gelatin: (+) liquid in 5 days
*Nitrate reduction: (+) red when added 2 indicators
*Citrate: (-) green
*Casein: (-) unclear
* EMB: (-) no growth
*MSA: (+) with yellow halo
*starch: (-) no clear around
2. Gram - bacillus
*tiny colonies
*yellow and sometimes red color inside of colony
*colony forming unit: Flocculent
*oxidase: (-) no blue
*Phenol Red
PR Glu: (A/-) Yellow
PR Lac: (-/-) No color change
PR Suc: (A/-) Yellow
*SIM test
Sulfur reducation: (-) No black
Indole: (-) no pink
motility: [+] little growth inside
*MR: (-) No color change
*VP: (+) red (red on the top and yellow on the bottom)
*Red blood cell Agar: alpha hemo
*MacConkey: G- and no color change (No fermentt lactose/noncoliform)
*DNAse: (+) clearing around
*Gelatin: (+) in 1 day
*Nitrate: (+) red after adding indicators
*Citrate: (+) blue
*Casein: (+) clear ........... (NOT SURE)
*EMB: poor growth
*MSA: (+) with yellow halo
*TSI: (A/A)(NG) yellow top and bottom, no gas
*Starch: (+) clear around after adding indicator
What is thename of Gram+ and Gram- Bacteria?
My instiructor gave us this bacteria list (he said someunknown bacteria can be not on the list)
| B. Cereus |
| E. coli |
| E. aerogenes |
| E. faecalis |
| Streptococcus bovis |
| K. pneumoniae (pneumoniae |
| S. aureus |
| S. epidermidis |
| Moraxella Moratella Osloensis |
| P. vulgaris |
| B. subtilis |
| M. luteus |
| P. aeruginosa |
| Bacillus Alvei |
| S. marcescens |
| A. faecalis |
| Staphylococcus Saprophyticus |
| Alcaligenes latus |
| Morganella morganii |
| S. sonnei |
| C. xerosis |
| Listeria innocua |
| Proteus mirabilis |
| Streptococcus agaloctiae |
| Salmonella Choleraesais |
| S. mitis |
