Question

1. Suppose you are purifying a protein on a weak cationexchanger (pH 7.5) and it comes off at 300mM salt. predict whatsalt concentraiton (lower, same, higher) the protein would come offif you used a strong cation exchanger. Explain the logic behindyour prediction in terms of the differences between aweak and strong exchanger.

2. Suppose you arepurifying a protein on a strong anion exchanger (pH6.5) and itcomes off at 0 mM salt. Predict where the protein will elute off ifyou were using a strong cation exchanger. Explain the logic behindyour prediction.