BIOB10H3 Study Guide - Glycosylation, Trypsin, Cell Membrane
6 Dec 2012
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Document Summary
Osteoclasts are a large multinucleate bone cell that absorbs bone tissue during growth and healing. Osteoblasts are cells that secrete the matrix for bone formation. Proteins are mainly studied by: determining primary structure, investigating secondary/tertiary structure, investigating protein localization, investigating protein function. When determining the primary structure of a protein, biologists either: extrapolate from genomic sequence, manually sequence the primary structure. During the process of genomic sequencing, biologists extrapolate amino acid sequence from gene/nucleotide sequence using computer database of genomes. Craig ventor was the founder of the celera genomics in 1999; he was able to create a invention to sequence the human genome faster then what the human genome. Project was doing but waited on the same day to release the sequence. Extrapolating from genomic sequence has 2 limitations/issues: These introns splices out of mrna and will not correctly predict protein. Introns intervening sequences that don"t code for anything sequence. Posttranslational modifications often cannot be predicted from genome.
