BIOB10Y3 Lecture Notes - Cell Plate, Synapsis, Insulin Receptor
Document Summary
Poly a tail plays a role in the degradation. Reaches 30 pts poly a nuclease is actively recruited, chops the tail up decapping enzyme is recruited. Active factors that shut off rna: produced by own cells called mirnas. Bind to a site in the 3" utr (untranslated regions through complementary base pairs) 1)interfere with translation active translation: inhibit after initiation- post initiation inhibiation, cause mrna to degrade inhibition of inti, mrna degradation, rna interference. C elegans (mello and fire) injected worms with sense rna, anti sense and double stranded rna. Double stranded rna taken up by cells and degraded the rna it was complement to but was specific. Figured out semi using radionucleotides: parental dna was incubated with heavy and transferred to light media. 1st generation all strands were hybrids heavy dna and light (parental and new) 2nd generation more and more light- more strands being made, hybrids don"t go away .
