Biology 2382B Lecture : Mitosis & Cell Cycle Control.docx
9 Dec 2012
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Lecture 7/8 - mitosis & cell cycle control (chp 18: p. 781-783) (chp 20: p. 847-853, p. 858-863, p. 887) Cell cycle - the ordered sequence of events in which a cell duplicates its chromosomes and divides into two genetically identical cells. In m phase (mitosis) most dramatic events can be observed microscopically. Difficult to determine where a cell is in interphase but easy when in mitosis . Facs can be used to quantify proportion of cells are in each cell cycle phase. This is achieved by measuring the intensity of fluorescence from the. Hoescht stain bound to dna at the end of s phase cell has double the. Dna and therefore fluorescence intensity is also doubled . Generalized growth and metabolism of the cell. Where most cells arrest when not dividing (i. e. enter go) Variable length (~11 hours in mammalian cells) Cell stops actively metabolizing during this pahase. Preparation for chromosome segregation and cell division (~4 hours)
