BIOL 300 Lecture Notes - Lecture 8: Tata Box, Taf1, Endonuclease
15 Dec 2012
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Document Summary
Recall gcn5, a hat which is part of a large multi-protein complex (saga or. Gcn4, a transcription factor in yeast is able to bind to the uas through a dna binding domain and its activation domain can bind a large multi-protein complex including gcn5. Remember, saga is used in the cell under stressful conditions by activating stress-response genes. Tbp associated factors: (tafs such as taf5, 6, 9, etc. : showing saga is like an alternative to tfiid, with some proteins in common. There are also subunits responsible for de-ubiquitination which bind to the nuclear pore, which might be how these complexes get near the nuclear pore to get more efficient mrna production during stress conditions. Towards the bottom, we have the hat activity, as well as an atpase function. The saga complex recognizes modifications made by: set1 methylase which works on k4, snf1 kinase which works on serine 10. These bring the saga"s gcn5 subunit closer to acetylate various lysines.
