PSY352H5 Lecture Notes - Lecture 5: Real-Time Polymerase Chain Reaction, Complementary Dna, Northern Blot
Document Summary
Ggene cloning single copy of gene placed in bacterium which replicates. The point of cloning is to create a large number of genes that makes it easier to analyze and manipulate. A single gene is just too small to manipulate. Special restriction enzyme that cut out particular genes to add to a plasmid (circular gene, vector that carries gene) Technology first utuilized 30 years ago in human medicine: human insulin first recombinant protein created, before the only insulin available for human use came from pigs. Now this method is used for testing effects of genes. Cdna = complementary dna, based upon mrna (not have introns, only has genes relevent to creating proteins etc [just coding region] therefore is much smaller and compacted) Study gene expression mostly by analyzing mrna levels in a sample based on hybridization. Microarray/dna chip small piece of glass (few square cm) that has. Other types of studying incl northern blot (most common) and quantitative.
