ANAT 262 Lecture Notes - Dna Construct, Electrophoresis, Sephadex

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Mouse experiment with feeding behaviors- goody related neurons in mice, which has a role in feeding behaviors. Heterologous: coming from different organisms or context. Channelredopsin is like the transgene that is introduced into neuron/cell type, they are introduced using promoters that drive expression in those particular neurons. Mouse is expressing channelredopsin in particular neurons, channel redopsin channels opens when pulses of shiny blue light is shined at it, allows ion flow and activation of depolarization of the neuron related to feeding behavior. How to induce particular behavior: isolating neurons, identifying neurons, isolating how we can activate them using chr. Structural characterisation links primary sequence to protein topology. Structure and function are not always revealed from primary sequence (amino acid sequence info)- impossible to predict how the thing is going to fold up in 3d shape. By determining protein structure we can better understand how proteins carry out their respective functions.

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