Biology 2382B Study Guide - Midterm Guide: Copii, Sox2, Intestinal Epithelium
31 Jan 2013
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First need to isolate + maintain cells in vitro. Then study how proteins drive biological processes. Technique for growing cells/tissues outside organism under strictly controlled conditions. Co2, o2: the tissue culture media contains ph indicator dye (phenol red, leave cells in incubator overnight. If the glass is charged, then cells will stick to the bottom. If you want cells in suspension then you need to keep in motion via magnetic spinner or roller bottles. If just left there then cells will naturally float to the bottom due to gravity. Primary cell culture: cells taken directly from organism, they divide a limited number of times, undergo contact inhibition. Cell line: cells that have been transformed and are thus immortal cells, grow indefinitely, do not undergo contact inhibition: hela was the first human cell line (no contact inhibition) caused cancer. Normal cells: elongated, aligned, orderly packed, grow in parallel arrays, contact inhibition to stop growing.
