BIOL 1111 Lecture Notes - Sigma Factor, Eif2, Operon

• School: University of Windsor
• Department: Biological Sciences
• Course: BIOL 1111
• Professor: Habetler

Document Summary

Initiation: protein assembly at origin: oric, a-t rich: helicase separate ds, topoisomerase, primase 10bp rna primer. Termination: ter region termination region pausing at fork: topoisomerase deconcatanate. Promoter: pribnow box -10, -35 sigma factor recognize specific promoter rna pol i. Elongation: promoter binding--> open complex formation -10 to +2 ternary complex: dna, pol, abortive initiation, make 10bp sigma fall off foot print shrink 30bp, pausing, driven by hydrolysis of ppi, more error prone than replication, bridge helix . Termination: regulated by attenuation not make unused stuff, rho independent: gc hairping puase, utail (at = less stable interaction) rho dependent: bind rut (rho utilization) hydrolysis of atp to pull of. 70s: 50s + 30s shine delgarno ribosome binding seq (multiple sites) have 5"and 3" utr. If1 + if3 block other site of small subunit psite open. Initiation: shine delgarno 6-8 poly purine , mrna bind shine delgarno, fmet -trna bind p site at aug.