BIOL 2905 Lecture Notes - Mycobacterium, Coulter Counter, Asepsis

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22 Feb 2013
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Content: patterns of metabolic pathways, vary with sources of carbon, energy and electrons or hydrogen atoms, -troph: suffix referring to eating, carbon. Heterotrophs: source is reduced organic compounds: energy. Chemotrophs: energy source if from redox reactions involving inorganic and organic compounds (aerobic respiration, anaerobic respiration, fermentation: electrons or hydrogen ions. Superoxide radical (o2 required to change superoxide radical into hydrogen peroxide and oxygen; anaerobes die because they lack this enzyme. Peroxidase: h2o2 into water and nad+ (oxidized) Hydroxyl radical: most reactive of the four; enzymes above iv. minimize production b. Types of organisms and need for oxygen i. ii. iii. iv. v. vi. vii. viii. Facultative anaerobes: can use anaerobic respiration or fermentation although prefer aerobic respiration; escherichia. Aerotolerant anaerobes: don"t use but can tolerate oxygen because they have some of the enzymes that detoxify it (often peroxidases since they don"t make more o2); lactobacilli. Microaerophiles: very limited ability to detoxify oxygen; can tolerate smaller amounts than aerotolerant anaerobes;

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