BIOL 202 Lecture Notes - Lecture 15: Sodium Hydroxide, Avidin, Biotin
25 Feb 2013
School
Department
Course
Professor
Document Summary
A pinched central region of kinetochore and centromere for division. The content-value paradox: the lack of correlation between genome size and the biological complexity of an organism. Satellite dnas: in prokaryotes, centrifugation of fragmented dna on a cesium chloride gradient produce a single band at a given density, irrespective of the size of the fragments. Use naoh to separate original strands, then bind with radioactive copies of dna. Most mouse dna are telocentric, the satellite dnas are located near the centromeres. Biotin will immediately bind to avidin, which comes from egg, a fluorescent dye is bound to the avidin, which allows for us to see where the telomere sequence is. Many repeated human sequences are derived from transposons, and many of them are inactivated and are no longer able to move.
